1- LOAD LIBRARIES and Prepare Slingshot object

2- CALCULATE TRAJECTORIES using slingshot from the UMAP coordinates.

3- Visualizing Slingshot-Inferred Lineage Paths on UMAP Using Minimum Spanning Tree (MST)

4- VISUALIZE ORIGINAL UMAP

5- Map the inferred trajectories (lineages) from the cluster-level graph into the actual reduced-dimensional space

6- Interactive 3D UMAP with Slingshot Lineages Colored by Cluster and Pseudotime

7- 3D Visualization of Slingshot-Inferred Lineages Colored by Cell Clusters in UMAP Space

8-Interactive 3D Visualization of Slingshot Trajectories Colored by Pseudotime in UMAP Space

9-Filter by Selecting significant genes

10-Visualize top 25 genes on heatmap

11- GENES CHANGING ALONG THE TRAJECTORIES

11a- EXPRESSION OF TEK w/ individual data points

11b- EXPRESSION OF TEK w/o individual data points

11c- Plotting Gene Expression of Tek (Tie2) on UMAP