1- LOAD LIBRARIES and Prepare Slingshot object
2- CALCULATE TRAJECTORIES using slingshot from the UMAP
coordinates.
3- Visualizing Slingshot-Inferred Lineage Paths on UMAP Using
Minimum Spanning Tree (MST)
4- VISUALIZE ORIGINAL UMAP
5- Map the inferred trajectories (lineages) from the cluster-level
graph into the actual reduced-dimensional space
6- Interactive 3D UMAP with Slingshot Lineages Colored by Cluster
and Pseudotime
7- 3D Visualization of Slingshot-Inferred Lineages Colored by Cell
Clusters in UMAP Space
8-Interactive 3D Visualization of Slingshot Trajectories Colored by
Pseudotime in UMAP Space
9-Filter by Selecting significant genes
10-Visualize top 25 genes on heatmap
11- GENES CHANGING ALONG THE TRAJECTORIES
11a- EXPRESSION OF TEK w/ individual data points
11b- EXPRESSION OF TEK w/o individual data points
11c- Plotting Gene Expression of Tek (Tie2) on UMAP
